Moroccan Watermelon Mosaic Virus in Squash Production Areas of Antalya Province
نویسندگان
چکیده
Bu çalışma Antalya ili kabak üretim alanlarından toplanan örneklerinde Fas karpuz mozayik virüsü (Moroccan watermelon mosaic virus; MWMV)’nün tespit edilmesi amacıyla 2019-2020 yıllarında yürütülmüştür. MWMV’nün varlığı serolojik ve moleküler yöntemlerle belirlenmiştir. Survey çalışmalarında virüs simptomu sergileyen 120 yaprak örneği alınmış, örneklere DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) yöntemi uygulanmıştır. testi sonucunda Aksu, Manavgat Kumluca ilçelerinden 9 örneğinde MWMV RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) enfekteli bu kullanılmıştır. uygulamasında kılıf protein geni için spesifik primerler kullanılarak yaklaşık 627 bç’lik bir kısım amplifiye edilmiş beklenen seviyede bant elde edilmiştir. çalışma, alanlarında MWMV’nin tanılanması bakımından ilk niteliğindedir.
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ژورنال
عنوان ژورنال: Uluda? Üniversitesi Ziraat Fakültesi dergisi
سال: 2022
ISSN: ['2651-4044', '1301-3165']
DOI: https://doi.org/10.20479/bursauludagziraat.943675